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World 1 · Inside a cell · 9 min

Knock out the power supply

A knock-out test with a control

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One way to find out what a part does is to remove it and see what fails: a knock-out experiment. Biologists do it with drugs and gene changes, and nature does it too: mature human red blood cells have no mitochondria and no nucleus. A fair test needs a control, the same cell under the same conditions with nothing removed. Mitochondria run aerobic respiration and make about 30 ATP from each glucose; without them, glycolysis in the cytoplasm still makes 2. Many other jobs, such as building proteins, making lipids and digesting waste, use ATP. So removing one part can have a direct effect and knock-on effects. Predict first, then run.

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A plant cell is kept in the dark and all its chloroplasts are knocked out. What does the one-minute test show?

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